Coding
Part:BBa_K3454042:Design
Designed by: Zhiyu Fang Group: iGEM20_ShanghaiTech_China (2020-10-27)
AsCas12a_RVR_6xHis_MBP
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1393
Illegal PstI site found at 4864
Illegal PstI site found at 5062 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2083
Illegal PstI site found at 1393
Illegal PstI site found at 4864
Illegal PstI site found at 5062 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 381
Illegal BglII site found at 5052 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1393
Illegal PstI site found at 4864
Illegal PstI site found at 5062 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1393
Illegal PstI site found at 4864
Illegal PstI site found at 5062
Illegal NgoMIV site found at 4596
Illegal AgeI site found at 3493
Illegal AgeI site found at 3964 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 79
Illegal BsaI.rc site found at 2236
Illegal BsaI.rc site found at 3898
Illegal SapI.rc site found at 1486
Design Notes
This is designed with MBP and his tag that can help AsCas12a mutant expression, folding and purification.
Source
It comes from expression of plasmid DNA(contains designed mutation ) of E.coli